Certification Board for Sterile Processing and Distribution (CBSPD) Sterile Processing Technician Practice Exam

What microorganism is used to biologically monitor the Ozone sterilizer?

1. Geobacillus stearothermophilus

2. Bacillus atrophaeus

3. Clostridium perfringens

4. Staphylococcus aureus

The correct answer is: Geobacillus stearothermophilus

The correct choice for the microorganism used to biologically monitor the Ozone sterilizer is Geobacillus stearothermophilus. This bacterium is a highly reliable indicator organism for steam and ozone sterilization processes. It is known for its ability to thrive at elevated temperatures, making it particularly suited for monitoring sterilization methods that operate under similar conditions. In biological monitoring, the selection of a suitable organism is critical for ensuring the efficacy of the sterilization cycle. Geobacillus stearothermophilus spores are resistant to certain environmental conditions, and their presence provides a clear benchmark for assessing whether the sterilization process was successful. Other microorganisms mentioned, while relevant to other forms of sterilization, do not serve the same purpose in the context of ozone sterilization. Bacillus atrophaeus is typically used for ethylene oxide sterilization processes. Clostridium perfringens is associated with anaerobic conditions and isn't a standard indicator for sterilization monitoring. Staphylococcus aureus, although significant in clinical contexts, is not employed in biological monitoring of sterilizers. Thus, Geobacillus stearothermophilus is the ideal choice for ensuring that Ozone sterilization processes are effective and capable of eliminating